Journal
/
Biologie
/
A Simple High Efficiency Protocol for Pancreatic Islet Isolation from Mice
Journal JoVE
Biologie
Un abonnement à JoVE est nécessaire pour voir ce contenu.  Connectez-vous ou commencez votre essai gratuit.
Journal JoVE Biologie
A Simple High Efficiency Protocol for Pancreatic Islet Isolation from Mice

A Simple High Efficiency Protocol for Pancreatic Islet Isolation from Mice

DOI:
10.3791/57048-v

•

06:56 min

•

August 30, 2019

•

, , , , ,
1Department of Nutrition and Food Science,Texas A&M University, 2Children’s Nutrition Research Center,Baylor College of Medicine

Chapitres

  • 00:04Titre
  • 00:34Pancreas Isolation
  • 02:50Islet Harvest
  • 05:58Results: Representative Fluorescence Cell-Imaged Islets
  • 06:19Conclusion

Summary

Traduction automatique

Traduction automatique

This islet isolation protocol described a novel route of collagenase injection to digest the exocrine tissue and a simplified gradient procedure to purify the islets from mice. It involves enzymatic digestion, gradient separation/purification, and islet hand-picking. Successful isolation can yield 250–350 high quality and fully functional islets per mouse.

Tags

Keywords: Pancreatic Islet IsolationHigh Efficiency ProtocolMouseCollagenase PBile DuctPancreatic DuctAmpulla Of VaterIslet YieldAbdominal IncisionDissecting MicroscopeMicro ForcepsMicro Serrefines

Article

Embed

AJOUTER À LA PLAYLIST

Usage Statistics

Vidéos Connexes

check_url/fr/57048?article_type=v

Murine Pancreatic Islet Isolation
check_url/fr/57048?article_type=v

Neutrophil Isolation Protocol
check_url/fr/57048?article_type=v

Human Pancreatic Islet Isolation: Part I: Digestion and Collection of Pancreatic Tissue
check_url/fr/57048?article_type=v

Human Pancreatic Islet Isolation: Part II: Purification and Culture of Human Islets
check_url/fr/57048?article_type=v

Isolation of Stem Cells from Human Pancreatic Cancer Xenografts
check_url/fr/57048?article_type=v

A Simple Protocol for Extracting Hemocytes from Wild Caterpillars
check_url/fr/57048?article_type=v

A Method for Mouse Pancreatic Islet Isolation and Intracellular cAMP Determination
check_url/fr/57048?article_type=v

Pancreatic Islet Embedding for Paraffin Sections
check_url/fr/57048?article_type=v

Imaging Calcium Dynamics in Subpopulations of Mouse Pancreatic Islet Cells
check_url/fr/57048?article_type=v

Isolation of High Quality Murine Atrial and Ventricular Myocytes for Simultaneous Measurements of Ca2+ Transients and L-Type Calcium Current

Read Article