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JoVE Journal
Genetics

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JoVE Journal Genetics
Generation of Genome-wide Chromatin Conformation Capture Libraries from Tightly Staged Early Drosophila Embryos
 

Generation of Genome-wide Chromatin Conformation Capture Libraries from Tightly Staged Early Drosophila Embryos

Article DOI: 10.3791/57001-v • 10:35 min • October 3rd, 2018
October 3rd, 2018 •
1, 1
1Max Planck Institute for Molecular Biomedicine

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This work describes a protocol for the generation of high resolution in situ Hi-C libraries from tightly staged pre-gastrulation Drosophila melanogaster embryos.

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Genome-wide Chromatin Conformation Capture Libraries Drosophila Embryos Chromatin Architecture Gene Expression Development High Resolution View Precisely Staged Embryos Transgenic Fly Libraries Formaldehyde Heptane PBST Glycerine Centrifugation
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