Journal
/
Neurosciences
/
In Vivo Single-Molecule Tracking at the Drosophila Presynaptic Motor Nerve Terminal
Journal JoVE
Neurosciences
Author Produced
This content is Free Access.
Journal JoVE Neurosciences
In Vivo Single-Molecule Tracking at the Drosophila Presynaptic Motor Nerve Terminal

In Vivo Single-Molecule Tracking at the Drosophila Presynaptic Motor Nerve Terminal

DOI:
10.3791/56952-v

•

06:45 min

•

January 14, 2018

•

, , , , ,
1Clem Jones Centre for Ageing Dementia Research, Queensland Brain Institute,The University of Queensland, 2VIB Centre for Brain and Disease Research, KU Leuven Department of Neurosciences,Leuven Institute for Neurodegenerative Disease (LIND), 3Queensland Brain Institute,The University of Queensland

Chapitres

  • 00:00Titre
  • 00:29Design of transgenic Drosophila
  • 00:44Dissection of third-instar Drosophila larva
  • 02:31Single particle tracking photo-activated localization microscopy
  • 04:31Data analysis
  • 05:13Representative results
  • 06:08Conclusion

Summary

Traduction automatique

Traduction automatique

Here we illustrate how single molecule photo-activated localization microscopy can be carried out on the motor nerve terminal of a live Drosophila melanogaster third instar larva.

Tags

Keywords: Single-molecule TrackingDrosophilaPresynaptic Motor Nerve TerminalIn VivoPhotoconvertible FluorophoreSchneider’s Insect MediumDissectionSylgard BaseTIRF MicroscopyNeuromuscular Junctions

Article

Embed

AJOUTER À LA PLAYLIST

Usage Statistics

Vidéos Connexes

check_url/fr/56952?article_type=v

In vivo Visualization of Synaptic Vesicles Within Drosophila Larval Segmental Axons
check_url/fr/56952?article_type=v

Patch-clamp Capacitance Measurements and Ca2+ Imaging at Single Nerve Terminals in Retinal Slices
check_url/fr/56952?article_type=v

Measuring Spinal Presynaptic Inhibition in Mice By Dorsal Root Potential Recording In Vivo
check_url/fr/56952?article_type=v

Acute Dissociation of Lamprey Reticulospinal Axons to Enable Recording from the Release Face Membrane of Individual Functional Presynaptic Terminals
check_url/fr/56952?article_type=v

Why Quantification Matters: Characterization of Phenotypes at the Drosophila Larval Neuromuscular Junction
check_url/fr/56952?article_type=v

Combined Recording of Mechanically Stimulated Afferent Output and Nerve Terminal Labelling in Mouse Hair Follicle Lanceolate Endings
check_url/fr/56952?article_type=v

Optical Monitoring of Living Nerve Terminal Labeling in Hair Follicle Lanceolate Endings of the Ex Vivo Mouse Ear Skin
check_url/fr/56952?article_type=v

Olfactory Behaviors Assayed by Computer Tracking Of Drosophila in a Four-quadrant Olfactometer
check_url/fr/56952?article_type=v

A Simple One-step Dissection Protocol for Whole-mount Preparation of Adult Drosophila Brains
check_url/fr/56952?article_type=v

3D Kinematic Analysis for the Functional Evaluation in the Rat Model of Sciatic Nerve Crush Injury

Read Article