Here we illustrate how single molecule photo-activated localization microscopy can be carried out on the motor nerve terminal of a live Drosophila melanogaster third instar larva.
Bademosi, A. T., Lauwers, E., Amor, R., Verstreken, P., van Swinderen, B., Meunier, F. A. In Vivo Single-Molecule Tracking at the Drosophila Presynaptic Motor Nerve Terminal. J. Vis. Exp. (131), e56952, doi:10.3791/56952 (2018).