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1Department of General Surgery, Tongren Hospital,Shanghai Jiao Tong University School of Medicine, 2Key Laboratory of Systems Biomedicine (Ministry of Education), Institute of Systems Biomedicine,Shanghai Jiao Tong University, 3Hongqiao International Institute of Medicine, Tongren Hospital,Shanghai Jiao Tong University School of Medicine
Chapitres
- 00:04Titre
- 01:01Affinity Purification of FLAG-tagged hEAG1 Channel Protein from HEK-293 Cells
- 04:18BCA Assay, Western Blotting, and Labeling the Purified Protein with Biotin for the BLI Assay
- 06:10Bio-layer Interferometry Assay
- 08:06Results: Interaction Kinetics of hEAG1 with PIP2 Using the Bio-layer Interferometry Assay
- 09:35Conclusion
El protocolo aquí describe las interacciones de la proteína de canal de iones hEAG1 purificada con la pequeña molécula lipídica ligando fosfatidilinositol 4, 5-bifosfato (PIP2). La medición demuestra que BLI podría ser un método potencial para la detección de iones de molécula pequeña novela canal ligando.
