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Imaging Mycobacterium tuberculosis in Mice with Reporter Enzyme Fluorescence
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Imaging Mycobacterium tuberculosis in Mice with Reporter Enzyme Fluorescence
Chapitres
- 00:04Titre
- 01:02Preparing the M. tuberculosis Inoculum
- 03:13Inoculating Mice with Airborne M. tuberculosis
- 05:33Reporter Enzyme Fluorescence Imaging and Analysis of Lung Homogenate
- 08:05Results: Imaging
- 09:32Conclusion
We describe the optical imaging of mice infected with Mycobacterium tuberculosis (M. tuberculosis) using reporter enzyme fluorescence (REF). This protocol facilitates the sensitive and specific detection of M. tuberculosis in pre-clinical animal models for pathogenesis, therapeutics and vaccine research.
