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完整蛋白质复合物的化学交联和质谱分析研究多亚基蛋白质组合体的体系结构

Journal JoVE
Biologie

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Journal JoVE Biologie

Combining Chemical Cross-linking and Mass Spectrometry of Intact Protein Complexes to Study the Architecture of Multi-subunit Protein Assemblies

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完整蛋白质复合物的化学交联和质谱分析研究多亚基蛋白质组合体的体系结构

19,205 Views

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10:01 min

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November 28, 2017

DOI:

10.3791/56747-v

DOI:

10.3791/56747-v

•

10:01 min

November 28, 2017

•

2 Views

Caroline Haupt*¹, Tommy Hofmann*¹, Sabine Wittig*¹, Susann Kostmann¹, Argyris Politis², Carla Schmidt¹

¹Interdisciplinary research center HALOmem,Martin Luther University Halle-Wittenberg, ²Department of Chemistry,Kings College London

Summary

蛋白质复合体的结构对其功能至关重要。结合各种质谱技术证明了研究其组装的强大功能。我们提供化学交联和本机质谱的协议, 并展示这些互补技术如何帮助阐明 multi-subunit 蛋白组件的结构。

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Chemical Cross-linking Mass Spectrometry Intact Protein Complexes Multi-subunit Protein Assemblies Structural Information Composition Connectivity Stoichiometry Protein Complex Purification Electrophoresis Coomassie Staining Protein Band Excision Disulfide Reduction Cysteine Alkylation Trypsin Digestion Peptide Extraction

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