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Respuesta auditiva del médula oblonga y la célula de pelo externa celulares parche abrazadera grabación en ratas postnatales
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1Department of Physiology, School of Basic Medical Sciences,Southern Medical University, 2School of Basic Medical Sciences,Guizhou Medical University, 3Department of Laboratory Medicines, Guangzhou General Hospital of Guangzhou Military Region,Southern Medical University, 4Experiment Teaching Center, School of Basic Medical Sciences,Southern Medical University
Chapitres
- 00:04Titre
- 00:56Auditory Brainstem Response Recording
- 02:12The Organ of Corti Dissection
- 03:22Immunofluorescence Staining
- 05:21Patch Clamp Recording of Isolated OHCs
- 07:33Results: Whole-Cell Patch Clamp Recording of Isolated Outer Hair Cells
- 08:19Conclusion
Este protocolo describe métodos para registrar la respuesta auditiva del médula oblonga de crías de rata postnatal. Para examinar el desarrollo funcional de las células de pelo externas, el procedimiento experimental de la abrazadera del remiendo de célula entera en aisladas células de pelo externas se describe paso a paso.
