This protocol describes an imaging-based method to activate T lymphocytes using photoactivatable peptide-MHC, enabling precise spatiotemporal control of T cell activation.
Sanchez, E., Huse, M. Spatial and Temporal Control of T Cell Activation Using a Photoactivatable Agonist. J. Vis. Exp. (134), e56655, doi:10.3791/56655 (2018).