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Snelle detectie van neurologische fenotypen in menselijke neurale voorlopercellen (NPC)
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Biologie du développement
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Journal JoVE Biologie du développement
Rapid Detection of Neurodevelopmental Phenotypes in Human Neural Precursor Cells (NPCs)
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Snelle detectie van neurologische fenotypen in menselijke neurale voorlopercellen (NPC)

9,804 Views

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10:47 min

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March 02, 2018

DOI:

10.3791/56628-v

DOI:
10.3791/56628-v

•

10:47 min
March 02, 2018

•

8 Views
, , , , , , , , ,
1Department of Neuroscience and Cell Biology,Rutgers Robert Wood Johnson Medical School, 2Center for Advanced Biotechnology and Medicine, Department of Neuroscience and Cell Biology,Rutgers Robert Wood Johnson Medical School, 3The Child Health Institute of NJ, Department of Obstetrics, Gynecology, and Reproductive Services,Rutgers Robert Wood Johnson Medical School, 4The Child Health Institute of NJ, Department of Neuroscience and Cell Biology,Rutgers Robert Wood Johnson Medical School, 5Department of Genetics,Rutgers University

Summary

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Neurologische processen zoals proliferatie, migratie en neurite uitvloeisel zijn vaak verstoord in neuropsychiatrische ziekten. Dus presenteren we protocollen om te snel en reproducibly beoordelen deze neurologische processen in menselijke iPSC afkomstige NPC's. Deze protocollen toestaan ook de beoordeling van de gevolgen van relevante factoren die de groei en therapeutics NPC ontwikkelingssamenwerking.

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Williams, M., Prem, S., Zhou, X., Matteson, P., Yeung, P. L., Lu, C., Pang, Z., Brzustowicz, L., Millonig, J. H., Dicicco-Bloom, E. Rapid Detection of Neurodevelopmental Phenotypes in Human Neural Precursor Cells (NPCs). J. Vis. Exp. (133), e56628, doi:10.3791/56628 (2018).

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