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Generation of Discriminative Human Monoclonal Antibodies from Rare Antigen-specific B Cells Circulating in Blood
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Journal JoVE Immunologie et infection
Generation of Discriminative Human Monoclonal Antibodies from Rare Antigen-specific B Cells Circulating in Blood

Generation of Discriminative Human Monoclonal Antibodies from Rare Antigen-specific B Cells Circulating in Blood

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13:14 min

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February 06, 2018

DOI:

10.3791/56508-v

DOI:
10.3791/56508-v

•

13:14 min
February 06, 2018

•

1 Views
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1CRCINA, Inserm, CNRS, Université d’Angers,Université de Nantes, 2LabEx IGO, “Immunotherapy, Graft, Oncology”, 3CHU Nantes, France

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Human Monoclonal AntibodiesAntigen-specific B CellsBloodDiscriminative AntibodiesImmunotherapyB LymphocytesDensity GradientMononuclear CellsTetramersMagnetic MicrobeadsCell Separation

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Devilder, M., Moyon, M., Saulquin, X., Gautreau-Rolland, L. Generation of Discriminative Human Monoclonal Antibodies from Rare Antigen-specific B Cells Circulating in Blood. J. Vis. Exp. (132), e56508, doi:10.3791/56508 (2018).

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