Journal
/
Bio-ingénierie
/
A Method for Determination and Simulation of Permeability and Diffusion in a 3D Tissue Model in a Membrane Insert System for Multi-well Plates
Journal JoVE
Bio-ingénierie
This content is Free Access.
Journal JoVE Bio-ingénierie
A Method for Determination and Simulation of Permeability and Diffusion in a 3D Tissue Model in a Membrane Insert System for Multi-well Plates

A Method for Determination and Simulation of Permeability and Diffusion in a 3D Tissue Model in a Membrane Insert System for Multi-well Plates

DOI:
10.3791/56412-v

•

10:33 min

•

February 23, 2018

•

, , , , , , ,
1Institute of Bioprocess and Biosystems Engineering,Hamburg University of Technology, 2Institute of Biotechnology, Department Medical Biotechnology,Technische Universität Berlin, 3TissUse GmbH

Chapitres

  • 00:04Introduction
  • 00:56Agarose Gel and Collagen Cell Model Preparation
  • 02:42Permeability Assay and Diffusion Simulation Setup
  • 05:54Parameter Optimization Addition
  • 08:05Results: Representative 3D Tissue Model Analyses
  • 09:30Conclusion

Summary

Traduction automatique

Traduction automatique

A method for determination of permeability in a membrane insert system for multi-well plates and in silico parameter optimization for the calculation of diffusion coefficients using simulation are presented.

Tags

Membrane Insert SystemPermeabilityDiffusionOrgan-on-a-chipAgarose GelCollagen Cell ModelKeratinocytesDonor SubstanceAcceptor SubstanceFluorescence Measurement

Article

Embed

AJOUTER À LA PLAYLIST

Usage Statistics

Vidéos Connexes

A Method for Ovarian Follicle Encapsulation and Culture in a Proteolytically Degradable 3 Dimensional System

Creating Two-Dimensional Patterned Substrates for Protein and Cell Confinement

Cellular Lipid Extraction for Targeted Stable Isotope Dilution Liquid Chromatography-Mass Spectrometry Analysis

Tissue Engineering of a Human 3D in vitro Tumor Test System

Systematic Analysis of In Vitro Cell Rolling Using a Multi-well Plate Microfluidic System

Generation of a Three-dimensional Full Thickness Skin Equivalent and Automated Wounding

Production, Characterization and Potential Uses of a 3D Tissue-engineered Human Esophageal Mucosal Model

Planar Gradient Diffusion System to Investigate Chemotaxis in a 3D Collagen Matrix

A Combined 3D Tissue Engineered In Vitro/In Silico Lung Tumor Model for Predicting Drug Effectiveness in Specific Mutational Backgrounds

Fabrication of Custom Agarose Wells for Cell Seeding and Tissue Ring Self-assembly Using 3D-Printed Molds

Read Article