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高分辨率荧光原位杂交在果蝇胚胎和组织中使用 Tyramide 信号放大

Journal JoVE
Génétique

This content is Free Access.

Journal JoVE Génétique

High Resolution Fluorescent In Situ Hybridization in Drosophila Embryos and Tissues Using Tyramide Signal Amplification

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高分辨率荧光原位杂交在果蝇胚胎和组织中使用 Tyramide 信号放大

12,498 Views

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14:21 min

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October 19, 2017

DOI:

10.3791/56281-v

DOI:

10.3791/56281-v

•

14:21 min

October 19, 2017

•

4 Views

Allison Jandura^1,2, Jack Hu¹, Ronit Wilk^1,2, Henry M. Krause^1,2

¹The Terrence Donnelly Centre for Cellular and Biomolecular Research,University of Toronto, ²Department of Molecular Genetics,University of Toronto

Summary

所描述的 rna原位杂交协议允许检测整个果蝇胚胎或解剖组织中的 rna。使用96井孔板和 tyramide 信号放大, 记录可以检测高分辨率, 灵敏度和吞吐量, 并以较低的成本。

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