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果蝇岛试验中运动行为的高通量分析
Journal JoVE
Neurosciences
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Journal JoVE Neurosciences
High-throughput Analysis of Locomotor Behavior in the Drosophila Island Assay
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果蝇岛试验中运动行为的高通量分析

8,554 Views

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10:30 min

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November 05, 2017

DOI:

10.3791/55892-v

DOI:
10.3791/55892-v

•

10:30 min
November 05, 2017

•

9 Views
, , , , ,
1Department of Human Genetics, Donders Institute for Brain, Cognition and Behaviour,Radboud University Medical Center, 2Centre for Molecular and Biomolecular Informatics, Radboud Institute for Molecular Life Sciences,Radboud University Medical Center, 3Department of Neurology, Donders Institute for Brain, Cognition and Behaviour,Radboud University Medical Center

Summary

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海岛试验是一种比较新的, cost-effective 的方法, 可用于评估的基本运动行为的果蝇。这份手稿描述了自动数据处理的算法和岛屿试验数据的客观量化, 使得这种方法成为一个敏感的高通量的大基因或药理屏幕的读数。

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Eidhof, I., Fenckova, M., Elurbe, D. M., van de Warrenburg, B., Castells Nobau, A., Schenck, A. High-throughput Analysis of Locomotor Behavior in the Drosophila Island Assay. J. Vis. Exp. (129), e55892, doi:10.3791/55892 (2017).

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