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High-throughput Analysis of Locomotor Behavior in the Drosophila Island Assay
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High-throughput Analysis of Locomotor Behavior in the Drosophila Island Assay
1Department of Human Genetics, Donders Institute for Brain, Cognition and Behaviour,Radboud University Medical Center, 2Centre for Molecular and Biomolecular Informatics, Radboud Institute for Molecular Life Sciences,Radboud University Medical Center, 3Department of Neurology, Donders Institute for Brain, Cognition and Behaviour,Radboud University Medical Center
Capítulos
- 00:05Título
- 01:03Preparations
- 03:19Island Assay and Data Collection
- 04:07Data Processing: Defining the Parameters to Run the Macro
- 06:37Data Processing: Running the “Drosophila Island Assay” Macro
- 08:55Results: Sample Experiment
- 09:37Conclusion
The island assay is a relatively new, cost-effective assay that can be used to evaluate the basic locomotor behavior of Drosophila melanogaster. This manuscript describes algorithms for automatic data processing and objective quantification of island assay data, making this assay a sensitive, high-throughput readout for large genetic or pharmacological screens.
