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与ChEC-seq的蛋白质-DNA相互作用的全基因组映射<em>酿酒酵母（Saccharomyces cerevisiae）</em
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Journal JoVE Génétique
Genome-wide Mapping of Protein-DNA Interactions with ChEC-seq in Saccharomyces cerevisiae
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与ChEC-seq的蛋白质-DNA相互作用的全基因组映射<em>酿酒酵母（Saccharomyces cerevisiae）</em

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10:43 min

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June 03, 2017

DOI:

10.3791/55836-v

DOI:
10.3791/55836-v

•

10:43 min
June 03, 2017

•

1 Views
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1Basic Sciences Division,Fred Hutchinson Cancer Research Center, 2Department of Biology,Indiana University

Summary

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我们描述了染色质内源性切割与高通量测序（ChEC-seq），染色质免疫沉淀（ChIP） - 正交法，用于在全基因组范围内用微球菌核酸酶（MNase）融合蛋白测定蛋白质结合位点。

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ChEC-seqProtein-DNA InteractionsGenome-wide MappingSaccharomyces CerevisiaeTranscriptionChromatinDNA BindingRegulatory FactorsHigh ResolutionLow BackgroundFormaldehyde CrosslinkingChromatin SolubilizationAntibodiesMNaseCalcium AdditionStop SolutionSDS

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Cite this Article

Grünberg, S., Zentner, G. E. Genome-wide Mapping of Protein-DNA Interactions with ChEC-seq in Saccharomyces cerevisiae. J. Vis. Exp. (124), e55836, doi:10.3791/55836 (2017).

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