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Protein-tRNA Agarose Gel Retardation Assays for Analysen af<em> N</em<sup> 6</sup> -threonylcarbamoyladenosin TcdA-funktion

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Biochimie

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Journal JoVE Biochimie

Protein-tRNA Agarose Gel Retardation Assays for the Analysis of the N⁶-threonylcarbamoyladenosine TcdA Function

Protein-tRNA Agarose Gel Retardation Assays for Analysen af<em> N</em<sup> 6</sup> -threonylcarbamoyladenosin TcdA-funktion

9,539 Views

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08:03 min

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June 21, 2017

DOI:

10.3791/55638-v

DOI:

10.3791/55638-v

•

08:03 min

June 21, 2017

•

8 Views

Francisco J. Fernández^2,3, Sara Gómez, Sergio Navas-Yuste, Miguel López-Estepa, M. Cristina Vega

¹Department of Physical Chemical Biology, Center for Biological Research (CIB-CSIC),Spanish National Research Council (CSIC), ²Department of Immunology,Complutense University School of Medicine, ³Abvance Biotech srl

Tags

Protein-tRNA Interaction Agarose Gel Retardation Assay RNA-binding Proteins TcdA Function RNA Biology Protein-RNA Complexes E. Coli BL21 DE3 PET-23D EC Triple-U IPTG Induction Cell Lysis Acid Phenol Extraction

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