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Full-cell Patch-clamp Gravações para determinação eletrofisiológica de Ion Selectivity em Channelrhodopsins
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Full-cell Patch-clamp Gravações para determinação eletrofisiológica de Ion Selectivity em Channelrhodopsins
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May 22, 2017
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This article describes how the ion selectivity of channelrhodopsin is determined with electrophysiological whole-cell patch-clamp recordings using HEK293 cells. Here, the experimental procedure for investigating chloride selectivity of an anion-selective channelrhodopsin is demonstrated. However, the procedure is transferable to other channelrhodopsins of distinct selectivity.
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Grimm, C., Vierock, J., Hegemann, P., Wietek, J. Whole-cell Patch-clamp Recordings for Electrophysiological Determination of Ion Selectivity in Channelrhodopsins. J. Vis. Exp. (123), e55497, doi:10.3791/55497 (2017).
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