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细胞迁移研究在垂直化学和氧梯度聚二甲基硅氧烷的聚碳酸酯微流体装置
Journal JoVE
Bio-ingénierie
This content is Free Access.
Journal JoVE Bio-ingénierie
Polydimethylsiloxane-polycarbonate Microfluidic Devices for Cell Migration Studies Under Perpendicular Chemical and Oxygen Gradients
DOI:

11:23 min

February 23, 2017

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Chapitres

  • 00:05Titre
  • 01:20Fabrication of the PC-embedded Top Layer
  • 02:59Assembly of the Device
  • 04:42Microfluidic Cell Migration Assay
  • 08:38Results: Characterization of Chemical and Oxygen Gradients Established within the Microfluidic Device, and Study of Cell Migration Under Gradient Combinations
  • 10:35Conclusion

Summary

Traduction automatique

The control of chemical and oxygen gradients is essential for cell cultures. This paper reports a polydimethylsiloxane-polycarbonate (PDMS-PC) microfluidic device capable of reliably generating combinations of chemical and oxygen gradients for cell migration studies, which can be practically utilized in biological labs without sophisticated instrumentation.

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