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Cell Lineage Analyses and Gene Function Studies Using Twin-spot MARCM
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Cell Lineage Analyses and Gene Function Studies Using Twin-spot MARCM
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March 02, 2017
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Here, we present a protocol for a mosaic labeling technique that permits the visualization of neurons derived from a common progenitor cell in two distinct colors. This facilitates neural lineage analysis with the capability of birth-dating individual neurons and studying gene function in the same neurons of different individuals.
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Shen, H., Hsu, T., Chung, P., Yu, H. Cell Lineage Analyses and Gene Function Studies Using Twin-spot MARCM. J. Vis. Exp. (121), e55278, doi:10.3791/55278 (2017).
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