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从人类脐带和胎盘胎儿分离和鉴定间充质干细胞

Journal JoVE
Biologie du développement

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Journal JoVE Biologie du développement

Isolation and Characterization of Mesenchymal Stromal Cells from Human Umbilical Cord and Fetal Placenta

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从人类脐带和胎盘胎儿分离和鉴定间充质干细胞

DOI:

10.3791/55224-v

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07:06 min

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April 03, 2017

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Naimisha Beeravolu², Christina McKee², Ali Alamri², Sasha Mikhael, Christina Brown², Mick Perez-Cruet⁴, G. Rasul Chaudhry²

¹Department of Biological Sciences,Oakland University, ²OU-WB Institute for Stem Cell and Regenerative Medicine, ³Department of Obstetrics and Gynecology,St. John Provindence – Providence Park Hospital, ⁴Department of Neurosurgery,Beaumont Health System

Chapitres

00:05Titre
01:09Human Umbilical Cord (UC), Cord-Placenta Junction (CPJ), and Fetal Placenta (FP) Processing and Cell Isolation
04:29Results: Colony-Forming Efficiency and Mesenchmyal Stromal Cell (MSC) Marker Expression of Perinatal Tissue-Isolated Cells
06:21Conclusion

Summary

Traduction automatique

在这里，我们提出了一个协议，用于人脐带（UC）和胎儿胎盘样品引入帘线衬（CL），脐带胶质（WJ），索胎盘结（CPJ），和胎儿胎盘（FP）为隔离的夹层以及使用该外植体培养技术间充质基质细胞（MSC）的表征。

Tags

Keywords: Mesenchymal Stromal Cells Umbilical Cord Fetal Placenta Stem Cell Biology Alternative Medicine Multi-potent Cord Mesenchymal Stromal Cells Anatomical Regions Umbilical Cord Wharton’s Jelly Epithelium Subamnion Blood Vessels Perivascular Jelly Trypsin Partial Digestion

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