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Biologie du développement
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A Versatile Mounting Method for Long Term Imaging of Zebrafish Development
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Biologie du développement
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Journal JoVE Biologie du développement
A Versatile Mounting Method for Long Term Imaging of Zebrafish Development

A Versatile Mounting Method for Long Term Imaging of Zebrafish Development

DOI:
10.3791/55210-v

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06:55 min

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January 26, 2017

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1Department of Developmental and Stem Cell Biology,Institut Pasteur, 2Department of Genetics,University of Cambridge, 3IBPS- Laboratoire de Biologie du Developpement (LBD),CNRS, UPMC, UMR 7622, INSERM ERL U1156

Chapitres

  • 00:05Titre
  • 00:43Embedding Embryos for Inverted or Upright Microscopy
  • 02:36Removal of Excess Agarose Around the Posterior Body
  • 04:03Mounting of Embryos for Vertical Light-sheet Microscope
  • 05:05Results: Long-term Time Lapse Imaging of Mounted Zebrafish Embryos
  • 06:15Conclusion

Summary

Traduction automatique

Traduction automatique

Here, we present a versatile mounting method that allows for the long-term time-lapse imaging of the posterior body development of live zebrafish embryos without perturbing normal development.

Tags

ZebrafishEmbryoLive ImagingMountingAgaroseDechorionationTricaineBiologie du développementMorphogenesisPosterior BodyOrientationCapillary NeedleMicrowellGlass-bottomed DishTransmitted Light

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