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A Versatile Mounting Method for Long Term Imaging of Zebrafish Development
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Biologie du développement
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Journal JoVE Biologie du développement
A Versatile Mounting Method for Long Term Imaging of Zebrafish Development

A Versatile Mounting Method for Long Term Imaging of Zebrafish Development

11,032 Views

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06:55 min

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January 26, 2017

DOI:

10.3791/55210-v

DOI:
10.3791/55210-v

•

06:55 min
January 26, 2017

•

1 Views
,
1Department of Developmental and Stem Cell Biology,Institut Pasteur, 2Department of Genetics,University of Cambridge, 3IBPS- Laboratoire de Biologie du Developpement (LBD),CNRS, UPMC, UMR 7622, INSERM ERL U1156

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Here, we present a versatile mounting method that allows for the long-term time-lapse imaging of the posterior body development of live zebrafish embryos without perturbing normal development.

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ZebrafishEmbryoLive ImagingMountingAgaroseDechorionationTricaineBiologie du développementMorphogenesisPosterior BodyOrientationCapillary NeedleMicrowellGlass-bottomed DishTransmitted Light

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Cite this Article

Hirsinger, E., Steventon, B. A Versatile Mounting Method for Long Term Imaging of Zebrafish Development. J. Vis. Exp. (119), e55210, doi:10.3791/55210 (2017).

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