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Biologie du développement
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Isolation of Mouse Endometrial Epithelial and Stromal Cells for In Vitro Decidualization
Journal JoVE
Biologie du développement
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Journal JoVE Biologie du développement
Isolation of Mouse Endometrial Epithelial and Stromal Cells for In Vitro Decidualization

Isolation of Mouse Endometrial Epithelial and Stromal Cells for In Vitro Decidualization

DOI:
10.3791/55168-v

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08:37 min

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March 02, 2017

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, ,
1Department of Development and Regeneration,Katholieke Universiteit Leuven (KU Leuven)

Chapitres

  • 00:05Titre
  • 00:58Uterine Isolation
  • 03:03Mouse Endometrial Epithelial Cell (MEEC) Culture
  • 04:59Mouse Endometrial Stromal Cell (MESC) Culture
  • 06:46Results: Decidualization of MEEC/MESC Cocultures
  • 08:05Conclusion

Summary

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Traduction automatique

This study presents a standardized and validated method for the isolation and culture of primary mouse endometrial stromal and epithelial cells, which can be used in a coculture system to study in vitro decidualization.

Tags

Mouse Endometrial CellsEndometrial Epithelial CellsEndometrial Stromal CellsIn Vitro DecidualizationCo-culture SystemParacrine SignalingReproductive MedicineVaginal SmearEstrus PhasePancreatinTrypsinCell IsolationCell Culture

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