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Immunologie et infection
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Legionella pneumophila membrana esterna Vescicole: Isolamento e analisi del loro potenziale pro-infiammatori sui macrofagi
Journal JoVE
Immunologie et infection
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Journal JoVE Immunologie et infection
Legionella pneumophila Outer Membrane Vesicles: Isolation and Analysis of Their Pro-inflammatory Potential on Macrophages
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Legionella pneumophila membrana esterna Vescicole: Isolamento e analisi del loro potenziale pro-infiammatori sui macrofagi

10,887 Views

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08:34 min

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February 22, 2017

DOI:

10.3791/55146-v

DOI:
10.3791/55146-v

•

08:34 min
February 22, 2017

•

9 Views
, , , , ,
1Institute for Lung Research, Universities of Giessen and Marburg Lung Center,Philipps-University Marburg, 2German Center for Lung Research, 3Department of Medicine, Pulmonary and Critical Care Medicine,University Medical Center Giessen and Marburg

Summary

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Qui, descriviamo la purificazione di Legionella pneumophila (L. pneumophila) vescicole membrana esterna (OMVS) da colture liquide. Tali vescicole purificate sono poi utilizzati per il trattamento dei macrofagi per analizzare il loro potenziale pro-infiammatoria.

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Cite this Article

Jung, A. L., Hoffmann, K., Herkt, C. E., Schulz, C., Bertrams, W., Schmeck, B. Legionella pneumophila Outer Membrane Vesicles: Isolation and Analysis of Their Pro-inflammatory Potential on Macrophages. J. Vis. Exp. (120), e55146, doi:10.3791/55146 (2017).

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