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Organ Culture and Whole Mount Immunofluorescence Staining of Mouse Wolffian Ducts
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Organ Culture and Whole Mount Immunofluorescence Staining of Mouse Wolffian Ducts
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January 13, 2017
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We present a method for isolation and culture of the mouse Wolffian duct (WD). We also demonstrate a detailed procedure for whole mount immunostaining of cultured/freshly isolated WDs with fluorescently tagged antibodies. Together, these techniques enable the study of WD development, coiling, and differentiation.
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Kumar, M., Tanwar, P. Organ Culture and Whole Mount Immunofluorescence Staining of Mouse Wolffian Ducts. J. Vis. Exp. (119), e55134, doi:10.3791/55134 (2017).
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