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生产和治疗间充质干行政/基质细胞(MSC)球体底漆在三维培养下无异物条件
Journal JoVE
Biologie du développement
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Journal JoVE Biologie du développement
Production and Administration of Therapeutic Mesenchymal Stem/Stromal Cell (MSC) Spheroids Primed in 3-D Cultures Under Xeno-free Conditions
DOI:

11:05 min

March 18, 2017

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Chapitres

  • 00:05Titre
  • 01:25MSC Expansion and Preparation of Activated MSC Spheroids in 3-D Hanging Drop Cultures
  • 04:50Intraperitoneal Delivery of Intact Spheroids
  • 08:02Results: Spheroids Produced Using MSC Hanging Drop Cultures
  • 09:31Conclusion

Summary

Traduction automatique

The therapeutic potential of mesenchymal stem/stromal cells (MSCs) is well-documented, however the best method of preparing the cells for patients remains controversial. Herein, we communicate protocols to efficiently generate and administer therapeutic spherical aggregates or 'spheroids' of MSCs primed under xeno-free conditions for experimental and clinical applications.

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