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Immunologie et infection
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单核细胞单层试验中用于评估Fcγ受体介导的吞噬作用
Journal JoVE
Immunologie et infection
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Journal JoVE Immunologie et infection
Use of a Monocyte Monolayer Assay to Evaluate Fcγ Receptor-mediated Phagocytosis
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单核细胞单层试验中用于评估Fcγ受体介导的吞噬作用

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06:27 min

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January 02, 2017

DOI:

10.3791/55039-v

DOI:
10.3791/55039-v

•

06:27 min
January 02, 2017

•

26 Views
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1Department of Laboratory Medicine and Pathobiology,University of Toronto, 2Centre for Innovation,Canadian Blood Services

Summary

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The monocyte monolayer assay (MMA) is an in vitro assay that utilizes isolated primary monocytes obtained from mammalian peripheral whole blood to evaluate Fcγ receptor (FcγR)-mediated phagocytosis.

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Monocyte Monolayer AssayFc Receptor-mediated PhagocytosisImmunohematologyBlood TransfusionAuto-antibodiesAllo-antibodiesRed Blood CellsOpsonizationPolyclonal Anti-D AntibodiesPBMCDensity Gradient CentrifugationIn Vitro Functional Assay

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Cite this Article

Tong, T. N., Branch, D. R. Use of a Monocyte Monolayer Assay to Evaluate Fcγ Receptor-mediated Phagocytosis. J. Vis. Exp. (119), e55039, doi:10.3791/55039 (2017).

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