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inoculant<em> Anopheles gambiae</em> Les moustiques avec perles à induire et mesurer la réponse immunitaire mélanisation
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Immunologie et infection
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Journal JoVE Immunologie et infection
Inoculating Anopheles gambiae Mosquitoes with Beads to Induce and Measure the Melanization Immune Response
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inoculant<em> Anopheles gambiae</em> Les moustiques avec perles à induire et mesurer la réponse immunitaire mélanisation

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08:24 min

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January 12, 2017

DOI:

10.3791/55013-v

DOI:
10.3791/55013-v

•

08:24 min
January 12, 2017

•

3 Views
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1Laboratoire d’écologie et d’épidémioloigie parasitaire, Institut de biologie,Université de Neuchâtel, 2Merkle Lab, Department of Entomology,Pennsylvania State University

Summary

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Through inoculation with beads, the described technique enables the stimulation of the mosquito melanization response in the hemolymph circulating system. The amount of melanin covering the beads can be measured after dissection as a measure of the immune response.

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Cite this Article

Barreaux, A. M. G., Barreaux, P., Thomas, M. B., Koella, J. C. Inoculating Anopheles gambiae Mosquitoes with Beads to Induce and Measure the Melanization Immune Response. J. Vis. Exp. (119), e55013, doi:10.3791/55013 (2017).

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