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Inoculating Anopheles gambiae Mosquitoes with Beads to Induce and Measure the Melanization Immune Response
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Immunologie und Infektion
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JoVE Journal Immunologie und Infektion
Inoculating Anopheles gambiae Mosquitoes with Beads to Induce and Measure the Melanization Immune Response

Inoculating Anopheles gambiae Mosquitoes with Beads to Induce and Measure the Melanization Immune Response

7,883 Views

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08:24 min

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January 12, 2017

DOI:

10.3791/55013-v

DOI:
10.3791/55013-v

•

08:24 min
January 12, 2017

•

3 Views
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1Laboratoire d’écologie et d’épidémioloigie parasitaire, Institut de biologie,Université de Neuchâtel, 2Merkle Lab, Department of Entomology,Pennsylvania State University

Summary

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Through inoculation with beads, the described technique enables the stimulation of the mosquito melanization response in the hemolymph circulating system. The amount of melanin covering the beads can be measured after dissection as a measure of the immune response.

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Anopheles GambiaeMelanizationImmune ResponseInoculationBeadsCapillary InjectionEcological ImmunologyMethyl GreenMosquito RearingInsect Dissection

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Cite this Article

Barreaux, A. M. G., Barreaux, P., Thomas, M. B., Koella, J. C. Inoculating Anopheles gambiae Mosquitoes with Beads to Induce and Measure the Melanization Immune Response. J. Vis. Exp. (119), e55013, doi:10.3791/55013 (2017).

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