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Experimental Protocol for Detecting Cyanobacteria in Liquid and Solid Samples with an Antibody Microarray Chip
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Experimental Protocol for Detecting Cyanobacteria in Liquid and Solid Samples with an Antibody Microarray Chip
Chapitres
- 00:05Titre
- 01:11Fluorescence Antibody Labeling
- 02:05CYANOCHIP Production
- 03:13Preparation of Environmental Multi-analyte Extracts for Fluorescent Sandwich Microarray Immunoassay
- 04:42Fluorescent Sandwich Microarray Immunoassay
- 05:20Incubation of Multi-analyte Sample Extract with Microarray and Washing
- 06:42Fluorescent Antibodies and Scanning Fluorescence
- 07:53Results: Cyanobacteria Detection Using the CYANOCHIP Multiplex Immunoassay
- 09:17Conclusion
The presence of cyanobacterial toxins in fresh water reservoirs for human consumption is a major concern for water management authorities. To evaluate the risk of water contamination, this article describes an protocol for the in-field detection of cyanobacterial strains in liquid and solid samples by using an antibody microarray chip.
Tags
CyanobacteriaAntibody MicroarrayFluorescence Microarray ImmunoassayEnvironmental MonitoringWater ManagementToxin DetectionBiodiversityAgricultural MonitoringPlanetary ExplorationSample ProcessingAntibody LabelingCYANOCHIPProtein Printing BufferTween 20Preimmune SerumBSA384-well MicroplatesPrinting RobotSlide Substrates
