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Experimental Protocol for Detecting Cyanobacteria in Liquid and Solid Samples with an Antibody Microarray Chip
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Chapitres
- 00:05Titre
- 01:11Fluorescence Antibody Labeling
- 02:05CYANOCHIP Production
- 03:13Preparation of Environmental Multi-analyte Extracts for Fluorescent Sandwich Microarray Immunoassay
- 04:42Fluorescent Sandwich Microarray Immunoassay
- 05:20Incubation of Multi-analyte Sample Extract with Microarray and Washing
- 06:42Fluorescent Antibodies and Scanning Fluorescence
- 07:53Results: Cyanobacteria Detection Using the CYANOCHIP Multiplex Immunoassay
- 09:17Conclusion
The presence of cyanobacterial toxins in fresh water reservoirs for human consumption is a major concern for water management authorities. To evaluate the risk of water contamination, this article describes an protocol for the in-field detection of cyanobacterial strains in liquid and solid samples by using an antibody microarray chip.
Tags
Keywords:
CyanobacteriaAntibody MicroarrayFluorescence Microarray ImmunoassayEnvironmental MonitoringWater ManagementToxin DetectionBiodiversityAgricultural MonitoringPlanetary ExplorationSample ProcessingAntibody LabelingCYANOCHIPProtein Printing BufferTween 20Preimmune SerumBSA384-well MicroplatesPrinting RobotSlide Substrates
