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Histological Quantification of Chronic Myocardial Infarct in Rats
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Journal JoVE Médecine
Histological Quantification of Chronic Myocardial Infarct in Rats

Histological Quantification of Chronic Myocardial Infarct in Rats

DOI:
10.3791/54914-v

•

09:45 min

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December 11, 2016

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, , , ,
1Cardiology, Department of Medicine,University of Fribourg

Chapitres

  • 00:05Titre
  • 01:15Heart Harvesting and Tissue Preparation
  • 04:07Masson-Goldner Trichrome Staining
  • 06:06Infarct Size Analysis
  • 08:19Results: Correlation between the Expansion Index (EI) and the Percentage of the Infarct Related to LV (A) or the Ejection Fraction
  • 09:14Conclusion

Summary

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The post-mortem assessment of myocardial infarction (MI) in rodents is based on quantification of the infarct on stained heart sections. We describe an accurate method to quantify the infarct size using systematic sampling of harvested rat hearts from base to apex and image analyses of trichrome-stained histological sections.

Tags

Keywords: Histological QuantificationChronic Myocardial InfarctRat ModelInfarct SizeLeft Ventricle RemodelingSternotomyLAD LigationTTC StainingInfarct AreaHealthy MyocardiumTransmural InfarctsNon-transmural Infarcts

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