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온도에 민감한 치명적인 돌연변이의 높은 처리량 로봇 보조 절연에<em> 클라 미도 모나스의 reinhardtii에서</em
Journal JoVE
Génétique
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Journal JoVE Génétique
High-Throughput Robotically Assisted Isolation of Temperature-sensitive Lethal Mutants in Chlamydomonas reinhardtii
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온도에 민감한 치명적인 돌연변이의 높은 처리량 로봇 보조 절연에<em> 클라 미도 모나스의 reinhardtii에서</em

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10:51 min

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December 05, 2016

DOI:

10.3791/54831-v

DOI:
10.3791/54831-v

•

10:51 min
December 05, 2016

•

3 Views
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1Laboratory of Cell Cycle Genetics,The Rockefeller University, 2Sainsbury Laboratory,University of Cambridge

Summary

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Temperature-sensitive (ts) lethal mutants are valuable tools to identify and analyze essential functions. Here we describe methods to generate and classify ts lethal mutants in high throughput.

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Chlamydomonas ReinhardtiiTemperature-sensitive Lethal MutantsHigh-throughput RoboticsUV MutagenesisEssential GenesPathwaysCell CycleLiquid DispenserPlateUV Lamp

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Breker, M., Lieberman, K., Tulin, F., Cross, F. R. High-Throughput Robotically Assisted Isolation of Temperature-sensitive Lethal Mutants in Chlamydomonas reinhardtii. J. Vis. Exp. (118), e54831, doi:10.3791/54831 (2016).

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