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JoVE Journal Genetics
Optogenetic Random Mutagenesis Using Histone-miniSOG in C. elegans
 

Optogenetic Random Mutagenesis Using Histone-miniSOG in C. elegans

Article DOI: 10.3791/54810-v • 04:51 min • November 14th, 2016
November 14th, 2016 •
1, 1,2
1Division of Biological Sciences, Section of Neurobiology, University of California in San Diego and Howard Hughes Medical Institute, 2Department of Cellular and Molecular Medicine, University of California in San Diego School of Medicine and Howard Hughes Medical Institute

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Genetically-encoded histone-miniSOG induces genome-wide heritable mutations in a blue light-dependent manner. This mutagenesis method is simple, fast, free of toxic chemicals, and well-suited for forward genetic screening and transgene integration.

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Optogenetic Random Mutagenesis Histone-miniSOG C. Elegans LED Set-up Mutagenize Genomic DNA Blue Light-dependent Proteins Reactive Oxygen Generator Proteins Advantages Of Technique Simple Mutagenesis Protocol Non-toxic Chemicals Mutagenesis Generator Heritable Mutations LED Illuminator Device Digital Function Generator BNC Cable LED Lights Custom Holder Blue Light Exposure Laser-protective Glasses Maximum Power Photometer Light-sensitive Strain
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