Journal
/
Chimie
/
An Optimized Protocol for the Efficient Radiolabeling of Gold Nanoparticles by Using a 125I-labeled Azide Prosthetic Group
Journal JoVE
Chimie
Un abonnement à JoVE est nécessaire pour voir ce contenu.  Connectez-vous ou commencez votre essai gratuit.
Journal JoVE Chimie
An Optimized Protocol for the Efficient Radiolabeling of Gold Nanoparticles by Using a 125I-labeled Azide Prosthetic Group

An Optimized Protocol for the Efficient Radiolabeling of Gold Nanoparticles by Using a 125I-labeled Azide Prosthetic Group

DOI:
10.3791/54759-v

•

07:23 min

•

October 10, 2016

•

, , , , , ,
1Advanced Radiation Technology Institute,Korea Atomic Energy Research Institute, 2Department of Radiation Biotechnology and Applied Radioisotope Science,University of Science and Technology

Chapitres

  • 00:05Titre
  • 01:08Radiosynthesis of the 125I-labeled Azide Prosthetic Group
  • 03:59Radiolabeling of Dibenzocyclooctyne-group-modified Gold Nanoparticles via the Copper-free Click Reaction
  • 05:53Results: Validation of the Purified Radiolabeled Products
  • 06:32Conclusion

Summary

Traduction automatique

Traduction automatique

A detailed procedure for the synthesis of a 125I-labeled azide and the radiolabeling of dibenzocyclooctyne (DBCO)-group-conjugated, 13-nm-sized gold nanoparticles using a copper-free click reaction is described.

Tags

Keyword Extraction:RadiolabelingGold Nanoparticles125I-labeled AzideStrain Promoted Copper Free Click ReactionRadiochemistryRadio Isotope LabeledImaging ProbesPET/SPECTRadiochemical YieldRadiochemical PurityRadio Iodination ReactionChloramine TSodium MetabisulfiteHPLC AnalysisReversed Phase Analytical Radio HPLCPreparative Radio HPLCRadiochemical Yield

Article

Embed

AJOUTER À LA PLAYLIST

Usage Statistics

Vidéos Connexes

check_url/fr/54759?article_type=v

Stabilizing Hepatocellular Phenotype Using Optimized Synthetic Surfaces
check_url/fr/54759?article_type=v

A Simple Method for the Size Controlled Synthesis of Stable Oligomeric Clusters of Gold Nanoparticles under Ambient Conditions
check_url/fr/54759?article_type=v

Preparation of a Corannulene-functionalized Hexahelicene by Copper(I)-catalyzed Alkyne-azide Cycloaddition of Nonplanar Polyaromatic Units
check_url/fr/54759?article_type=v

A Protocol for the Production of Gliadin-cyanoacrylate Nanoparticles for Hydrophilic Coating
check_url/fr/54759?article_type=v

A Simple and Efficient Protocol for the Catalytic Insertion Polymerization of Functional Norbornenes
check_url/fr/54759?article_type=v

Rapid Nanoprobe Signal Enhancement by In Situ Gold Nanoparticle Synthesis
check_url/fr/54759?article_type=v

Luminophore Formation in Various Conformations of Bovine Serum Albumin by Binding of Gold(III)
check_url/fr/54759?article_type=v

Synthesis and Characterization of Amphiphilic Gold Nanoparticles
check_url/fr/54759?article_type=v

Synthesis of Aptamer-PEI-g-PEG Modified Gold Nanoparticles Loaded with Doxorubicin for Targeted Drug Delivery
check_url/fr/54759?article_type=v

Quantitative SERS Detection of Uric Acid via Formation of Precise Plasmonic Nanojunctions within Aggregates of Gold Nanoparticles and Cucurbit[n]uril

Read Article