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基于CFSE-试验鼠皮肤树突状细胞的迁移习进引流淋巴结在与感染<em>牛分枝杆菌</em>卡介苗
Journal JoVE
Immunologie et infection
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Journal JoVE Immunologie et infection
A CFSE-based Assay to Study the Migration of Murine Skin Dendritic Cells into Draining Lymph Nodes During Infection with Mycobacterium bovis Bacille Calmette-Guérin
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基于CFSE-试验鼠皮肤树突状细胞的迁移习进引流淋巴结在与感染<em>牛分枝杆菌</em>卡介苗

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07:14 min

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October 09, 2016

DOI:

10.3791/54620-v

DOI:
10.3791/54620-v

•

07:14 min
October 09, 2016

•

5 Views
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1Department of Microbiology, Tumor and Cell Biology (MTC),Karolinska Institutet

Summary

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An assay in mice to track cell migration from skin to draining lymph node is described which enables the characterization of skin Dendritic cells mobilized to the lymph node after footpad infection with Bacille Calmette-Guérin.

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CFSEDendritic CellsMigrationDraining Lymph NodesMycobacterium Bovis BCGFoot Pad InjectionCell TrackingFlow Cytometry

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Cite this Article

Bollampalli, V. P., Nylén, S., Rothfuchs, A. G. A CFSE-based Assay to Study the Migration of Murine Skin Dendritic Cells into Draining Lymph Nodes During Infection with Mycobacterium bovis Bacille Calmette-Guérin. J. Vis. Exp. (116), e54620, doi:10.3791/54620 (2016).

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