Journal
/
Biologie du développement
/
تطبيق الحواجز غير نفاذ جنبا إلى جنب مع المرشح عامل الخرز غارقة لدراسة إشارات الاستقرائي في الفرخ
Journal JoVE
Biologie du développement
This content is Free Access.
Journal JoVE Biologie du développement
Application of Impermeable Barriers Combined with Candidate Factor Soaked Beads to Study Inductive Signals in the Chick
Please note that all translations are automatically generated. Click here for the English version.

تطبيق الحواجز غير نفاذ جنبا إلى جنب مع المرشح عامل الخرز غارقة لدراسة إشارات الاستقرائي في الفرخ

9,103 Views

•

08:04 min

•

November 17, 2016

DOI:

10.3791/54618-v

DOI:
10.3791/54618-v

•

08:04 min
November 17, 2016

•

2 Views
,
1Randall Division of Cell and Molecular Biophysics,King’s College London, Guy’s Campus

Summary

Automatically generated

Protocols using impermeable barriers to block induction events between tissues of the main body axis and flank in the chick embryo required for limb formation are described. Beads soaked in candidate inductive signals are used to overcome the effect of barrier placement and analysis of gene expression confirms this.

Explore More Videos

Impermeable BarrierCandidate FactorLimb InductionChick EmbryoBiologie du développementSurgical InterventionSignaling MoleculesLateral Plate MesodermGene ExpressionInductive SignalsLimb Bud InitiationFoil BarrierAir SacEggshellInner Cell Membrane

Read Article

Cite this Article

Wilde, S., Logan, M. P. Application of Impermeable Barriers Combined with Candidate Factor Soaked Beads to Study Inductive Signals in the Chick. J. Vis. Exp. (117), e54618, doi:10.3791/54618 (2016).

Download .ris file

Copy

Share Video

.

Copy