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通过酶联凝集素测定来测量神经氨酸酶流感抑制抗体滴度
Journal JoVE
Immunologie et infection
This content is Free Access.
Journal JoVE Immunologie et infection
Measuring Influenza Neuraminidase Inhibition Antibody Titers by Enzyme-linked Lectin Assay

通过酶联凝集素测定来测量神经氨酸酶流感抑制抗体滴度

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08:11 min

September 06, 2016

DOI:

08:11 min
September 06, 2016

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Summary

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We describe the enzyme-linked lectin assay (ELLA) for measuring influenza neuraminidase (NA)-inhibition antibody titers in sera. The assay uses peanut agglutinin to quantify galactose residues that become accessible when NA removes sialic acid from fetuin-coated, 96-well plates.

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