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1Davee Department of Neurology and Clinical Neurosciences,Feinberg School of Medicine, Northwestern University, 2Center for Molecular Innovation and Drug Discovery,Northwestern University, 3Department of Pharmacology,Feinberg School of Medicine, Northwestern University, 4High Throughput Analysis Laboratory, Department of Molecular Biosciences,Northwestern University, 5Department of Physiology,Feinberg School of Medicine, Northwestern University
Chapitres
- 00:05Titre
- 01:08High Throughput Screen
- 03:07Confirmation of Hits Using TAMRA-tagged HCN1 Peptide
- 04:33Dose-response Validation of Bead-based Proximity Assay
- 07:15Results: Identification of Small Molecule Inhibitors Between HCN1 and TRIPb8 Interactions
- 09:14Conclusion
A interacção entre os canais HCN e a sua sub-unidade auxiliar tem sido identificada como um alvo terapêutico em Perturbação Depressiva Major. Aqui, um método baseado em polarização de fluorescência para identificar pequenas moléculas inibidoras desta interacção proteína-proteína, é apresentada.
