需要订阅 JoVE 才能查看此. 登录或开始免费试用。
Method for Identifying Small Molecule Inhibitors of the Protein-protein Interaction Between HCN1 and TRIP8b
8,361 Views
•
10:20 min
•
November 11, 2016
DOI:
1Davee Department of Neurology and Clinical Neurosciences,Feinberg School of Medicine, Northwestern University, 2Center for Molecular Innovation and Drug Discovery,Northwestern University, 3Department of Pharmacology,Feinberg School of Medicine, Northwestern University, 4High Throughput Analysis Laboratory, Department of Molecular Biosciences,Northwestern University, 5Department of Physiology,Feinberg School of Medicine, Northwestern University
Summary
Automatically generated
The interaction between HCN channels and their auxiliary subunit has been identified as a therapeutic target in Major Depressive Disorder. Here, a fluorescence polarization-based method for identifying small molecule inhibitors of this protein-protein interaction, is presented.
Read Article
Cite this Article
Han, Y., Lyman, K. A., Clutter, M., Schiltz, G. E., Ismail, Q., Cheng, X., Luan, C., Chetkovich, D. M. Method for Identifying Small Molecule Inhibitors of the Protein-protein Interaction Between HCN1 and TRIP8b. J. Vis. Exp. (117), e54540, doi:10.3791/54540 (2016).
Copy