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“吞噬体封闭试验”可视化吞噬体形成的三个维度利用全内反射荧光显微镜（TIRFM）
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Immunologie et infection
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Journal JoVE Immunologie et infection
“Phagosome Closure Assay” to Visualize Phagosome Formation in Three Dimensions Using Total Internal Reflection Fluorescent Microscopy (TIRFM)
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“吞噬体封闭试验”可视化吞噬体形成的三个维度利用全内反射荧光显微镜（TIRFM）

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10:07 min

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August 26, 2016

DOI:

10.3791/54470-v

DOI:
10.3791/54470-v

•

10:07 min
August 26, 2016

•

2 Views
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1Inserm U1016, Institut Cochin, CNRS UMR 8104,Université Paris Descartes, Sorbonne Paris Cité

Summary

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We describe an experimental setup to visualize with unprecedented high resolution phagosome formation and closure in three dimensions in living macrophages, using total internal reflection fluorescence microscopy. It allows monitoring of the base of the phagocytic cup, the extending pseudopods, as well as the precise site of phagosome scission.

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Cite this Article

Marie-Anaïs, F., Mazzolini, J., Bourdoncle, P., Niedergang, F. "Phagosome Closure Assay" to Visualize Phagosome Formation in Three Dimensions Using Total Internal Reflection Fluorescent Microscopy (TIRFM). J. Vis. Exp. (114), e54470, doi:10.3791/54470 (2016).

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