This article describes a simple and reproducible protocol to manipulate dissolved oxygen conditions in a laboratory setting for animal behavior studies. This protocol may be used in both teaching and research laboratory settings to evaluate organismal response of macroinvertebrates, fishes, or amphibians to changes in dissolved oxygen concentration.
Grant, C. J., McLimans, C. J. A Simple Approach to Manipulate Dissolved Oxygen for Animal Behavior Observations. J. Vis. Exp. (112), e54430, doi:10.3791/54430 (2016).