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Biologie du développement
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イメージングは​​、単一細胞の解像度で胚および出生後の心をクリア
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Biologie du développement
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Journal JoVE Biologie du développement
Imaging Cleared Embryonic and Postnatal Hearts at Single-cell Resolution
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イメージングは​​、単一細胞の解像度で胚および出生後の心をクリア

8,022 Views

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07:30 min

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October 07, 2016

DOI:

10.3791/54303-v

DOI:
10.3791/54303-v

•

07:30 min
October 07, 2016

•

2 Views
, , , , , , , ,
1Department of Molecular and Cellular Physiology,Albany Medical College, 2Department of Neuroscience and Experimental Therapeutics,Albany Medical College

Summary

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We describe a protocol to volumetrically image fluorescent protein labeled cells deep inside intact embryonic and postnatal hearts. Utilizing tissue-clearing methods in combination with whole mount staining, single fluorescent protein-labeled cells inside an embryonic or postnatal heart can be imaged clearly and accurately.

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Single-cell ResolutionCardiac DevelopmentFluorescent ProteinPECAMAlexa Fluor 647DAPICUBICSCALEEmbryonic HeartPostnatal HeartGene FunctionProtein FunctionImagingConfocal Microscopy

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Cite this Article

Shaikh Qureshi, W. M., Miao, L., Shieh, D., Li, J., Lu, Y., Hu, S., Barroso, M., Mazurkiewicz, J., Wu, M. Imaging Cleared Embryonic and Postnatal Hearts at Single-cell Resolution. J. Vis. Exp. (116), e54303, doi:10.3791/54303 (2016).

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