/

Immunologie et infection

/

一种简单有效的方法构建突变牛痘病毒载体

Journal JoVE
Immunologie et infection

This content is Free Access.

Journal JoVE Immunologie et infection

A Simple and Efficient Approach to Construct Mutant Vaccinia Virus Vectors

Please note that all translations are automatically generated. Click here for the English version.

一种简单有效的方法构建突变牛痘病毒载体

10,804 Views

•

09:16 min

•

October 30, 2016

DOI:

10.3791/54171-v

DOI:

10.3791/54171-v

•

09:16 min

October 30, 2016

•

2 Views

Ming Yuan*¹, Pengju Wang*^2,3, Louisa S. Chard¹, Nicholas R. Lemoine^1,2,3, Yaohe Wang^1,2,3

¹Center for Molecular Oncology, Barts Cancer Institute,Queen Mary University of London, ²Sino-British Research Centre for Molecular Oncology, National Center for International Research in Cell and Gene Therapy,Zhengzhou University, ³School of Basic Medical Sciences, Academy of Medical Sciences,Zhengzhou University

Summary

牛痘病毒（VV）已被广泛用于生物医学研究和人体健康的改善。本文介绍使用CRISPR-Cas9系统编辑VV基因组的简单，高效的方法。

Explore More Videos

CRISPR-Cas9 Mutant Vaccinia Virus Viral Vector Gene Therapy Vaccination CV-1 Cells Transfection Shuttle Donor Vector Plaque Purification RFP-positive Plaques Cell Lysis Virus Dilution

Read Article