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القائم HPLC الفحص لمراقبة خارج الخلية النكليوتيد / نوكليوزيد الأيض في خلايا سرطان الدم الليمفاوي المزمن الإنسان
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HPLC-based Assay to Monitor Extracellular Nucleotide/Nucleoside Metabolism in Human Chronic Lymphocytic Leukemia Cells
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القائم HPLC الفحص لمراقبة خارج الخلية النكليوتيد / نوكليوزيد الأيض في خلايا سرطان الدم الليمفاوي المزمن الإنسان

10,844 Views

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11:29 min

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July 20, 2016

DOI:

10.3791/54124-v

DOI:
10.3791/54124-v

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11:29 min
July 20, 2016

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5 Views
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1Department of Medical Sciences, Human Genetics Foundation (HuGeF),University of Turin

Summary

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The protocol described here represents an easy and reproducible method that employs reverse phase high-performance liquid chromatography (RP-HPLC) to measure purine metabolism on chronic lymphocytic leukemia (CLL) cells cultured under different conditions.

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HPLCNucleotide MetabolismNucleoside MetabolismChronic Lymphocytic LeukemiaCLL CellsPurine MetabolismAdenosineExtracellular EnvironmentCell CultureDensity Gradient CentrifugationPBMCMagnetic BeadsCell Purification

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Cite this Article

Serra, S., Deaglio, S. HPLC-based Assay to Monitor Extracellular Nucleotide/Nucleoside Metabolism in Human Chronic Lymphocytic Leukemia Cells. J. Vis. Exp. (113), e54124, doi:10.3791/54124 (2016).

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