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Immunologie et infection
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A Rapid Strategy for the Isolation of New Faustoviruses from Environmental Samples Using Vermamoeba vermiformis
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Immunologie et infection
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Journal JoVE Immunologie et infection
A Rapid Strategy for the Isolation of New Faustoviruses from Environmental Samples Using Vermamoeba vermiformis

A Rapid Strategy for the Isolation of New Faustoviruses from Environmental Samples Using Vermamoeba vermiformis

7,520 Views

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09:27 min

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June 04, 2016

DOI:

10.3791/54104-v

DOI:
10.3791/54104-v

•

09:27 min
June 04, 2016

•

1 Views
, , ,
1Faculty of Medicine and Pharmacy, Research Unit for Infectious and Tropical Emerging Diseases,Aix Marseille University, 2Pole of Infectious and Tropical Diseases, Clinical and Biological Sector, Federation of Bacteriology-Hygiene Virology,University Hospital Institute Mediterranean Infection

Summary

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We describe here the latest advances in viral isolation for the characterization of new genotypes of Faustovirus, a new asfarvirus-related lineage of giant viruses. This protocol can be applied to the high throughput isolation of viruses, especially giant viruses infecting amoeba.

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FaustovirusesVermamoeba VermiformisVirus IsolationEnvironmental SamplesAntibiotic And Antifungal MixtureStarvation MediumFlow CytometryAmoeba CultureVirus ProspectingPan-genomeVirology

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Cite this Article

Bou Khalil, J. Y., Andreani, J., Raoult, D., La Scola, B. A Rapid Strategy for the Isolation of New Faustoviruses from Environmental Samples Using Vermamoeba vermiformis. J. Vis. Exp. (112), e54104, doi:10.3791/54104 (2016).

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