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Biologie du développement
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Prediction and Validation of Gene Regulatory Elements Activated During Retinoic Acid Induced Embryonic Stem Cell Differentiation
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Biologie du développement
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Journal JoVE Biologie du développement
Prediction and Validation of Gene Regulatory Elements Activated During Retinoic Acid Induced Embryonic Stem Cell Differentiation

Prediction and Validation of Gene Regulatory Elements Activated During Retinoic Acid Induced Embryonic Stem Cell Differentiation

DOI:
10.3791/53978-v

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09:07 min

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June 21, 2016

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, , ,
1Sanford-Burnham-Prebys Medical Discovery Institute at Lake Nona, 2Department of Biochemistry and Molecular Biology,Research Center for Molecular Medicine, Medical and Health Science Center, University of Debrecen, 3MTA-DE “Lendulet” Immunogenomics Research Group,University of Debrecen

Chapitres

  • 00:05Titre
  • 00:45Enhancer Selection Based on ChIP-seq Analysis
  • 01:27Transfection of Embryonic Stem Cells
  • 03:21β-Galactosidase and Luciferase Assays
  • 05:19Characterization of Enhancer RNA and Measurement of eRNA Transcription
  • 06:34Results: Retinoic Acid Induced Enhancer Activation in Embryonic Stem Cells
  • 08:13Conclusion

Summary

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Traduction automatique

In this work we provide an experimental workflow of how active enhancers can be identified and experimentally validated.

Tags

Gene Regulatory ElementsEnhancersRetinoic AcidEmbryonic Stem Cell DifferentiationReporter Plasmid ConstructTransfectionTranscription Regulation

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