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이미지 Zebrafish의 눈 개발에 가벼운 시트 형광 현미경을 사용하여
Journal JoVE
Biologie du développement
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Journal JoVE Biologie du développement
Using Light Sheet Fluorescence Microscopy to Image Zebrafish Eye Development
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이미지 Zebrafish의 눈 개발에 가벼운 시트 형광 현미경을 사용하여

33,301 Views

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13:01 min

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April 10, 2016

DOI:

10.3791/53966-v

DOI:
10.3791/53966-v

•

13:01 min
April 10, 2016

•

23 Views
, , , , ,
1Max Planck Institute of Molecular Cell Biology and Genetics, 2HHMI Janelia Research Campus, 3Berlin Institute of Medical Systems Biology of the Max Delbrück Center

Summary

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Light sheet fluorescence microscopy is an excellent tool for imaging embryonic development. It allows recording of long time-lapse movies of live embryos in near physiological conditions. We demonstrate its application for imaging zebrafish eye development across wide spatio-temporal scales and present a pipeline for fusion and deconvolution of multiview datasets.

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Cite this Article

Icha, J., Schmied, C., Sidhaye, J., Tomancak, P., Preibisch, S., Norden, C. Using Light Sheet Fluorescence Microscopy to Image Zebrafish Eye Development. J. Vis. Exp. (110), e53966, doi:10.3791/53966 (2016).

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