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Efficient Nucleic Acid Extraction and 16S rRNA Gene Sequencing for Bacterial Community Characterization
 

Efficient Nucleic Acid Extraction and 16S rRNA Gene Sequencing for Bacterial Community Characterization

Article DOI: 10.3791/53939-v • 12:37 min • April 14th, 2016
April 14th, 2016 •
1, 1, 1
1Ragon Institute of MGH, MIT, and Harvard, Massachusetts General Hospital

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We describe an efficient, robust, and cost effective method for extracting nucleic acid from swabs for characterization of bacterial communities using 16S rRNA gene amplicon sequencing. The method allows for a common processing approach for multiple sample types and accommodates a number of downstream analytic processes.

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Nucleic Acid Extraction 16S RRNA Gene Sequencing Bacterial Community Characterization DNA Isolation RNA Isolation Swabs Human Sample Bacterial Composition Host Characteristics Disease Status Vaginal Bacterial Communities Body Sites Ectocervical Swabs Wet Ice Nucleic Acid Extraction Preparation Hood Cleaning Bead Beading Tube NaCl-Tris-EDTA Buffer SDS Phenol Chloroform Isoamyl Alcohol
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