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A Miniaturized Glycan Microarray Assay for Assessing Avidity and Specificity of Influenza A Virus Hemagglutinins
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Immunologie et infection
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Journal JoVE Immunologie et infection
A Miniaturized Glycan Microarray Assay for Assessing Avidity and Specificity of Influenza A Virus Hemagglutinins

A Miniaturized Glycan Microarray Assay for Assessing Avidity and Specificity of Influenza A Virus Hemagglutinins

7,810 Views

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10:32 min

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May 29, 2016

DOI:

10.3791/53847-v

DOI:
10.3791/53847-v

•

10:32 min
May 29, 2016

•

5 Views
, ,
1Department of Cell and Molecular Biology, Chemical Physiology and Microbial Science,The Scripps Research Institute, 2Department of Medicinal Chemistry and Chemical Biology, Utrecht Institute for Pharmaceutical Sciences,Utrecht University

Summary

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Using a printed glycan microarray strategy, a conventional 96-well plate assay was miniaturized for analysis of influenza A virus hemagglutinin avidity and specificity for sialic acid containing receptors.

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Glycan MicroarrayInfluenza A VirusHemagglutininReceptor BindingAviditySpecificityGlycan Sample PreparationPrinting BufferPAA-conjugated GlycansMonovalent GlycansAmine-functionalized Dyes384-well Microtiter PlateArrayerPoly-L-lysine Coated SlidesHumidity Control

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McBride, R., Paulson, J. C., de Vries, R. P. A Miniaturized Glycan Microarray Assay for Assessing Avidity and Specificity of Influenza A Virus Hemagglutinins. J. Vis. Exp. (111), e53847, doi:10.3791/53847 (2016).

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